Ouabain Affects the Expression of Activation Markers, Cytokine Production, and Endocytosis of Human Monocytes

Figure 3

Time course of ouabain effect over the expression of activation and costimulatory molecules by monocytes. Cells were labeled with specific antibodies for 30 minutes (see Section 2) after 12, 24, and 48 hours of culture in the absence or presence of 10⁻⁷ M of ouabain. (a) Histograms showing the patterns of CD69, CD80, HLA-DR, and CD86 expression on monocytes untreated (empty histograms) or treated with 10⁻⁷ M of ouabain (gray histograms) for 12, 24, and 48 hours. 24 hours, 𝑛≥6; 12 and 48 hours, 𝑛=1, with the exception of CD69 experiments (𝑛≥3). (b) Data are expressed as the percentage of CD69⁺ monocytes and lines refer to the median of three (12-hour experiments) or four (24- and 48-hour experiments) independent measurements from distinct individuals. ^∗𝑃<0.05 using two-tailed paired 𝑡-test.

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