Ouabain Affects the Expression of Activation Markers, Cytokine Production, and Endocytosis of Human Monocytes
Figure 7
Effect of ouabain on dextran-FITC endocytosis and CD206 expression. Cells were cultured in the absence or presence of 10−7 M of ouabain for 24 hours. After 24 hours, these cells were incubated for 1 hour with FITC-conjugated dextran (1 mg/mL) for endocytic assays or incubated for 30 minutes with anti-CD206 PE-Cy5 to evaluate CD206 expression. Fluorescence signals were measured by flow cytometry. (a) Representative experiment showing the patterns of dextran uptake. Other results are expressed as (b) the mean of fluorescence intensity of dextran-FITC ± SEM and (c) the percentage of monocytes engaged in endocytosis—dextran-FITC+ cells ± SEM. (d) The mean fluorescence intensity of CD206 ± SEM and (e) the percentage of CD206+ cells ± SEM. Data are representative of seven endocytic activity assays and four flow cytometric analysis of CD206 expression. using two-tailed paired -test.
