Potent Anti-Inflammatory Activity of Pyrenocine A Isolated from the Marine-Derived Fungus Penicillium paxilli Ma(G)K
Figure 2
Anti-inflammatory effects of pyrenocine A on the production of NO. Pretreatment procedure: cells were pretreated with different concentrations of pyrenocine A (3.75–0.11 μM) for 2 h and then stimulated with LPS (1 μg/mL) during 18 h. Posttreatment procedure: cells were stimulated with LPS (1 μg/mL) for 2 h and then pyrenocine A was added (3.75–0.11 μM) during 18 h. Afterwards, the supernatants were collected, and nitrite concentrations were determined using Griess reagent. Results indicate the mean ± SEM for six independent experiments performed in quadruplicate for each condition. * versus medium (control), # versus LPS (ANOVA followed by Bonferroni). ND not detectable.
