Potent Anti-Inflammatory Activity of Pyrenocine A Isolated from the Marine-Derived Fungus Penicillium paxilli Ma(G)K
Figure 5
Effects of pyrenocine A on the expression of Mac-1 and B7.2 by macrophages. Pretreatment procedure: cells were pretreated with different concentrations of pyrenocine A (3.75–0.94 μM) for 2 h and then stimulated with LPS (1 μg/mL) during 18 h. (b) Posttreatment procedure: cells were stimulated with LPS (1 μg/mL) for 2 h and then pyrenocine A was added (3.75–0.94 μM) during 18 h. The expression of surface receptors such as Mac-1 and B7.1 was evaluated by MIF and by measuring the percentage of positive cells, respectively. All samples were analyzed using flow cytometry and a FACSCanto machine with BD FACSDiva software. Results are representative of three independent experiments.
