Potent Anti-Inflammatory Activity of Pyrenocine A Isolated from the Marine-Derived Fungus Penicillium paxilli Ma(G)K
Figure 6
Anti-inflammatory effects of pyrenocine A by TLR9/MyD88 pathway. Pretreatment procedure: cells were pretreated with different concentrations of pyrenocine A (3.75–0.11 μM) for 2 h and then stimulated with CpG (1 μg/mL) during 18 h (a) or Poly I:C (1 μg/mL) (c). Posttreatment procedure: cells were stimulated with CpG (1 μg/mL) (b) or Poly I:C (1 μg/mL) (d) for 2 h and then pyrenocine A (3.75–0.11 μM) was added during 18 h. The supernatants were collected to quantify NO. Results correspond to the mean ± SEM of three independent experiments performed in quintuplicate for each condition. # versus CpG or Poly I:C alone (ANOVA).
