Decreased Inflammatory Responses of Human Lung Epithelial Cells after Ethanol Exposure Are Mimicked by Ethyl Pyruvate

Figure 9

Effects of ethanol (EtOH), ethyl pyruvate (EtP), or sodium pyruvate (NaP) on the adhesiveness of neutrophils to lung epithelial cells A549 after IL-1beta (a) or lipopolysaccharide (LPS) stimulation. Cells were treated with EtOH (low dose, LoD = 85 mM and high dose, HiD = 170 mM), EtP (LoD = 2.5 mM and HiD = 10 mM), or NaP (10 mM) for 1 h and then stimulated with IL-1beta (1 ng/mL) or LPS (1 μg/mL) for 24 h. After the incubation periods, neutrophils were added and the adhesion capacity after 30 minutes was analyzed (given as % of total neutrophils). The data are presented as means ± s.e.m. versus untreated but stimulated control, ctrl; versus untreated and unstimulated cells.

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