Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus

Table 2

Primers and cycling parameters for real time RT-PCR detection of PU.1 and IL-9.

Genes	Gene bank accession	Primer sequences (5′ to 3′)	Tm (°C)	Expected product sizes
PU.1	NM_003120.2	F: GGAGCCCGGCTGGATGTTAC	60.04	79 bp
		R: CACCAGGTCTTCTGATGGCTGA	60.03
IL-9	NM_000590.1	F: CTCTAGCAGTCCACTTCACCAA	59.96	112 bp
		R: ACAGCATGGGTCTGTCTTCT	58.64
GATA-3	NM_001002295.1	F: AGCATGAAGCTGGAGTCGTC	60.6	141 bp
		R: ACAGTTCACACACTCCCTGC	58.7
IRF-4	NM_002460	F: GACCCGCAGATGTCCATGAG	60.53	82 bp
		R: TGTAGTTGTGAACCTGCTGGG	60.2
𝛽-Actin	NM_001101.3	F: TAGGCGGACTGTTACTGAGC	59.18	233 bp
		R: TGCTCCAACCAACTGCTGTC	60.82

Note. F: forward; R: reverse.