Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
Figure 2
EtOH-containing liquid diet affects fatty liver and increased proinflammatory IL-6 release 4 weeks after feeding mice an ethanol (EtOH-fed) or control (pair-fed) Lieber DeCarli diet; blood samples and livers were harvested as described in Section 2. Chow-fed animals served as internal controls for the pair feeding approach. Data are given as . versus all. In (a), liver body ratio from pair-fed mice is presented. Hepatic dry fat content was quantified by means of Soxhlet technique as described in Section 2. section (b). Serum aspartate aminotransferase levels were measured (c). Representative photomicrographs of HE stained liver sections from (d) pair- and (e) ethanol-fed mice are presented. Bar equals 100 μm. In (f), systemic levels of IL-6, and in (g), hepatic IL-6 proteins are shown. versus pair-fed Ctrl.
