Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
Figure 4
Effect of chronic ethanol on hepatic topography of cis-NF- transcriptional induction and hepatic macrophage activation of NF-κB dependent EGFP expression was analyzed using fluorescence microscopy in livers harvested from cis-NF-κ mice and prepared as described in Section 2. Representative liver lobes from pair-fed ((a), (c), (e), and (g)) and ethanol-fed ((b), (d), (e), and (h)) mice are shown. Green fluorescence by EGFP represents NF-κB activity ((a), (b): bar equals 100 μm). Additional GFP antibody staining identifies the topography of NF-κB activation ((c), (d): bar equals 50 μm; (e), (f): bar equals 200 μm). The encircled areas mark central veins; the crosses mark portal fields. Immunostaining for F4/80 (red fluorescence) identifies Kupffer cells in cis-NF-κ mice livers; overlay images show colabeled cells, marked by arrows ((g), (h): bar equals 20 μm).
