Activation of Adenosine A3 Receptor Alleviates TNF-α-Induced Inflammation through Inhibition of the NF-κB Signaling Pathway in Human Colonic Epithelial Cells
Figure 3
Expression of A3AR mRNA and protein in HT-29 cells. HT-29 cells were treated with various concentrations (10 nM, 30 nM, and 50 nM) of 2-Cl-IB-MECA for 30 min and then incubated with TNF-α (10 ng/mL) for 6 h in order to perform mRNA expression assays by qRT-PCR or 18 h for protein expression measurements by western blot. (a, b) Compared with the NC group, the mRNA and protein expression levels of A3AR showed no significant alterations in other groups (). (c, d) Representative pictures of A3AR protein expression in indicated groups detected by western blot. Data are shown as the mean ± SD from three independent experiments.
