Development and Validation of Protein Microarray Technology for Simultaneous Inflammatory Mediator Detection in Human Sera

Figure 4

Correlation between microarray and ELISA cytokine detection levels. The levels of 16 cytokines were quantified by microarray and ELISA techniques in up to 30 human serum samples. Paired -tests showed there were no significant differences between the two techniques for any of the cytokines tested. The 16 cytokines were eotaxin-1, eotaxin-2, IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-17, IL-23, IP-10, MCP-1, RAGE, TGF-β, TNF-α, and VEGF.

(a) IL-6

(b) IL-4

(c) IL-8

(d) IL-1β

(e) TNF-α

(f) IL-10

(g) VEGF

(h) IL-17

(i) Eotaxin-1

(j) TGF-β

(k) MCP-1

(l) IFN-γ

(m) RAGE

(n) IL-23

(o) IP-10

(p) Eotaxin-2