Splenic Stromal Cells from Aged Mice Produce Higher Levels of
IL-6 Compared to Young Mice
Figure 2
IL-6 protein induced by LPS in CD45− stromal cells and IL-6 mRNA expression level in fresh stromal cells. (a) Splenic CD45− stromal cells from young and aged mice were cultured with 0, 1, and 10 μg/mL LPS for 24 hours, and IL-6 in the supernatant was measured by ELISA. *P < 0.05. The data are the representative of two similar experiments (). (b) IL-6 mRNA of freshly isolated splenic CD45− stromal cells from young and aged mice () was measured by quantitative RT-PCR. The results are expressed as mean ± SD. The average normalized IL-6 mRNA level in aged mice was set at 1.0. *P < 0.05.
