Splenic Stromal Cells from Aged Mice Produce Higher Levels of
IL-6 Compared to Young Mice
Figure 5
IL-6 and IL-17 mRNA expression levels in whole spleen cells and CD45− stromal cells in vivo after LPS intraperitoneal administration for 1.5 h. (a) IL-6 mRNA of freshly isolated whole spleen cells from young and aged mice () was measured by quantitative RT-PCR after LPS injection (3.3 μg/g body weight). The average normalized IL-6 mRNA level in aged mice was set at 1.0. (b) IL-6 mRNA of splenic CD45− stromal cells from young and aged mice () sacrificed 1.5 h after injection was measured by quantitative RT-PCR. The average normalized IL-6 mRNA level in aged mice was set at 1.0. *P < 0.05. (c) IL-17 mRNA and (d) IL-1β of freshly isolated whole spleen cells from young and aged mice () were measured by quantitative RT-PCR after LPS injection. The average normalized IL-17 mRNA level in aged mice was set at 1.0. The results are expressed as mean ± SD.
