Quercetin Protects against Obesity-Induced Skeletal Muscle Inflammation and Atrophy

Figure 4

Quercetin suppresses the expression of atrophic factors in cocultured myotubes and macrophages treated with FFA. C2C12 Mb were cocultured with M⌀ for 24 h and then treated with 500 μM FFA for the next 24 h. Quercetin (50 μM) was added for 1 h before coculture started, and DMSO was used as control. (a) qRT-PCR analysis of transcripts of atrophic genes, including Atrogin-1 and MuRF1. (b) Expressions of MuRF1 and α-tubulin were determined by Western blotting. The intensities of the bands were measured using ImageJ software. Data are means ± SEM of three independent triplicate experiments. for the differences between the Co/FFA group and the Mix, Mix/FFA, or Co groups. and for the differences between the Co/FFA/Q and Co/FFA groups.

(a)

(b)