Inhibition of Macrophage Functions by the C-Terminus of Murine S100A9 Is Dependent on B-1 Cells
Figure 3
Phagocytosis assays by bone marrow-derived macrophages (BMDM), cocultivated with B-1 cells, treated with mS100A9p. BMDM adhered to coverslips (1 × 105 cells/well) were incubated in the presence of B-1 cells (1 × 105 cells/well) concomitant with mS100A9p (0.59; 1.17; or 2.35 M in RPMI-1640 medium), for 1 h at 37°C in 5% CO2. Coculture assays were evaluated with (a) or without (b) direct contact of these two cell populations, using transwell chambers. Experimental controls were cocultures of BMDM and B-1 cells incubated only with culture medium. After 1 h, both cocultures were incubated with 1 × 106 Candida albicans particles for 1 h. Results are expressed as means ± SD, using five samples per group. ≤ 0.05, when compared to the control group (RPMI).
(a) Phagocytic activity of BMDM cocultivated with B-1 cells, with direct contact
(b) Phagocytic activity of BMDM cocultivated with B-1 cells, without direct contact
