Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis
Figure 2
Representative flow cytometry plots. Blood leukocytes (a) were defined by gating on CD45-positive cells. The percentage of Ly6G-positive neutrophils from an individual representative mouse per group is listed. For analyzing the lymphocyte population in MLNs (b), MLN cells were first gated to exclude debris. Numbers indicate the percentage of CD3ε-positive lymphocytes in MLN cells. For analyzing the expression of adhesion molecules and chemokine receptors by T cells (c), lymphocytes were first identified based on low FSC and SSC characteristics. CD4- or CD8-positive lymphocytes were gated to analyze the percentages of adhesion molecule- and chemokine receptor-expressing CD4+ and CD8+ lymphocytes. Representative dot plots of leukocytes in blood are shown.
