Glutamine Supplementation Attenuates Expressions of Adhesion Molecules and Chemokine Receptors on T Cells in a Murine Model of Acute Colitis

Table 2

Leukocyte populations in blood and mesenteric lymph nodes (MLNs) (%).

	C	G	DC	DG
Blood
Neutrophils	8.7±0.5	8.0±0.9	16.1±2.7^∗	16.9±2.1^∗
Monocytes	7.2±0.9	6.7±0.7	8.2±1.3	7.7±1.5
T cells	11.9±0.4	11.7±0.4	9.3±1.2	9.3±1.4
B cells	50.3±0.9	53.4±3.1	53.2±2.7	49.0±2.1
Th cells	6.7±1.6	6.4±0.4	7.3±1.2	8.1±0.8
Tc cells	3.6±0.8	4.4±0.3	4.5±0.2	4.1±0.3
MLNs
T cells	45.2±2.4	46.7±2.2	32.1±2.3^∗	34.9±1.0^∗
B cells	30.3±1.8	29.0±3.3	31.3±2.1	29.7±1.4
Th cells	11.8±0.3	12.3±0.7	11.2±0.9	12.1±0.9
Tc cells	6.3±0.4	6.7±0.6	5.8±0.3	6.5±0.5

CD45-positive cells were considered to be leukocytes and gated to determine the population of leukocytes using a flow cytometer. Staining for Ly-6G, F4/80, CD3𝜀, and CD19 was used to respectively identify neutrophil, monocyte, T cell, and B cell populations. For the analysis of T cell subpopulations, lymphocytes were gated on the basis of their forward-and side-scatter profiles. Percentages of T helper (Th) and cytotoxic T (Tc) cells were respectively determined by CD4-and CD8-expressing cells in lymphocytes. Values are presented as the mean ± SEM. *Significantly differs from the C group (𝑃<0.05).