Toll-Like Receptor 4 in Bone Marrow-Derived Cells Contributes to the Progression of Diabetic Retinopathy
Figure 2
Ultrastructural signs of the blood retina barrier. (a to d) Representative electron micrographs of the retinal capillary ultrastructure from the inner layer are shown. (a) Capillary from the WT/WT (donor/recipient) group, showing a thick BM (0.18 μm, arrowhead) with pericyte edema (); inset, monocyte infiltration in the retinal tissue (★). (b) Capillary from the WT/Mut group. Protrusion of some villi into the lumen in endothelial cells (arrow). Reduced thickening of the basal membrane (0.15 μm, arrowhead). (c) Capillary from the Mut/WT group, showing a relatively thin BM (0.12 μm, arrowhead). Part of the crest of the mitochondria is shortened (arrow); inset, the obvious swelling of the mitochondrial crest. (d) Capillary from the Mut/Mut group, showing a thin BM (0.10 μm, arrowhead). The structure of the mitochondria is intact (arrow, magnification is indicated by the bar in the figure, mice/group). (e) Vascular basement membrane thickness was measured in 12 retinal vessels from each group (from three mice). Basement membrane thickness was measured at 5 locations around the perimeter of the vessel and averaged to obtain a value for each vessel. , WT/WT group compared with the Mut/WT group, #, WT/WT group compared with the Mut/Mut group, and Δ, WT/WT group compared with the WT/Mut group.
