Rapamycin Attenuated Cardiac Hypertrophy Induced by Isoproterenol and Maintained Energy Homeostasis via Inhibiting NF-κB Activation

Table 1

Primer sequences for qRT-PCR.

Target gene	Sequence
ANF	Forward: 5′-GGAAGTCAACCCGTCTCA-3′
	Reverse: 5′-AGCCCTCAGTTTGCTTTT-3′
BNP	Forward: 5′-TTTGGGCAGAAGATAGACCG-3′
	Reverse: 5′-AGAAGAGCCGCAGGCAGAG-3′
𝛽-MHC	Forward: 5′-GACAACGCCTATCAGTACATG-3′
	Reverse: 5′-TGGCAGCAATAACAGCAAAA-3′
GAPDH	Forward: 5′-AGGAGTAAGAAACCCTGGAC-3′
	Reverse: 5′-CTGGGATGGAATTGTGAG-3′
CPT-1𝛽	Forward: 5′-TCAAGGTTTGGCTCTATGAGGGCT-3′
	Reverse: 5′-TCCAGGGACATCTTGTTCTTGCCA-3′
CPT-2	Forward: 5′-TCCTGCATACCAGCAGATGAACCA-3′
	Reverse: 5′-TATGCAATGCCAAAGCCATCAGGG-3′
MCAD	Forward: 5′-CTGCTCGCAGAAATGGCGATGAAA-3′
	Reverse: 5′-CAAAGGCCTTCGCAATAGAGGCAA-3′
LCAD	Forward: 5′-AATGGGAGAAAGCCGGAGAAGTGA-3′
	Reverse: 5′-GATGCCGCCATGTTTCTCTGCAAT-3′
IL-1𝛽	Forward: 5′-TCCTCTGTGACTCGTGGGAT-3′
	Reverse: 5′-TCAGACAGCACGAGGCATTT-3′
IL-2	Forward: 5′-CCAAGCAGGCCACAGAATTG-3′
	Reverse: 5′-TCCAGCGTCTTCCAAGTGAA-3′
TNF-𝛼	Forward: 5′-TGGCGTGTTCATCCGTTCTC-3′
	Reverse: 5′-CCCAGAGCCACAATTCCCTT-3′
PGC-1𝛼	Forward: 5′-ACGAAAGGCTCAAGAGGGACGAAT-3′
	Reverse: 5′-CACGGCGCTCTTCAATTGCTTTCT-3′
PPAR𝛼	Forward: 5′-AGCTCAGGACACAAGACGTTGTCA-3′
	Reverse: 5′-AGGGACTTTCCAGGTCATCTGCTT-3′
𝛽-actin	Forward: 5′-TCGTGCGTGACATTAAAGAG-3′
	Reverse: 5′-ATTGCCGATAGTGATGACCT-3′