Uteroglobin, a Possible Ligand of the Lipoxin Receptor Inhibits Serum Amyloid A-Driven Inflammation
Figure 3
SAA-induced IL-8 levels were significantly downregulated in HEC-1A cells overexpressing UG. (a) Reverse transcription-PCR of ALX mRNA in HEC-1A cells and ALX-antisense (AS-ALX) oligo-treated HEC-1A cells. The result is from a representative gel of two performed. (b) SAA-dependent IL-8 release as measured by ELISA, in HEC-1A cells, is inhibited when pretreated with ALX-antisense oligo. In cells SAA-induced IL-8 release is significantly lower in comparison to HEC-1A. Student’s -test for SAA versus AS-ALX treated HEC-1A cells or cells was calculated, (**). Data were normalized using number of cells. Results are the mean ± SD of three separate experiments with quadruplicate determinations.
