NF-κB activation modulates LPS-induced iNOS expression by peritoneal macrophages. To demonstrate the importance of NF-κB activation on LPS-induced iNOS expression, the macrophage culture was incubated with two different NF-κB inhibitors [pyrrolidine dithiocarbamate (PDTC) and dexamethasone (DEX)] (100 and 500 μM) for 30 min before the addition of LPS (1 μg/mL) (time zero). The culture supernatant was collected for nitrite quantification by the Griess method (a), while the iNOS synthesis was determined in cell lysates by ELISA (b). Data is a representative of three independent experiments with similar results. Values are expressed as means ± S.E.M. 4–10 samples per group. * versus saline and # versus LPS group.
