CCK-2R does not alter LPS-induced iNOS expression by peritoneal macrophages. The relevance of CCK-2R was pharmacologically tested, incubating macrophage culture with selective antagonist [YM-022 (CCK-2R antagonist; 10−9–10−6 M)] for 30 min before the addition of CCK at 10−10 or 10−6 M. Thirty minutes later, cells were stimulated by LPS (1 μg/mL) (time zero). At the indicated time-points, the culture supernatant was collected for nitrite quantification by Griess method (a and b), while the iNOS synthesis was determined in cell lysates by ELISA (c). Data is a representative of three independent experiments with similar results. Values are expressed as means ± S.E.M. 4–12 samples per group. * versus saline, # versus LPS, and a versus respective CCK group.
