Intestinal Mucosal Barrier Is Injured by BMP2/4 via Activation of NF-B Signals after Ischemic Reperfusion
Figure 3
(a) Western blotting determined the expression of phosphorylated NF-B in IEC-6 after treatment with BMP2 and BMP4 for 6 h. The phosphorylated NF-B significantly increased compared with the control group, versus control. Noggin partially decreased NF-B transcriptional activity. , different from a single treatment with BMP2 or BMP4. (b) Immunofluorescence detected the translocation of NF-B to the nucleus after treatment with BMP2 and BMP4 for 30 min, and noggin partially reversed the nuclear localization of NF-B. (c) The fluorescence intensity of phosphorylated NF-B was significantly increased in the I/R group compared to the sham group. In the I/R group, NF-B exhibited significant nuclear localization in the distal villus, where abundant BMP2 and BMP4 are secreted after I/R (as shown in Figure 1(c)). The abundant BMP2 and BMP4 directly activated NF-B resulting in its nuclear localization, and noggin decreased the nuclear localization of NF-B in the I/R + noggin group. (d) Western blotting detected ERK expression upon BMP2 and BMP4 treatment at the defined time points. Phosphorylated ERK1/2 expression progressively increased in a time-dependent manner.
