Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity

Table 3

Colonic mucosal mRNA expression in Wt and Fat-1 TNBS-treated mice¹.

Gene	Wt	Fat-1
IL-17A	6.54 ± 2.95	2.04 ± 0.73*
IL-17F	6.14 ± 1.33	5.96 ± 1.03
IL-21	0.63 ± 0.09	0.15 ± 0.07*
IL-22	0.48 ± 0.17	0.95 ± 0.32
IL-23	1.63 ± 0.42	1.01 ± 0.41
IL-23R	1.72 ± 0.44	1.76 ± 0.28
IL-27	3.68 ± 0.84	7.50 ± 1.49*
CCL20	4.93 ± 1.59	4.25 ± 0.61
CCR6	0.45 ± 0.97	0.7 ± 0.48
ROR𝛾𝜏	15.79 ± 1.29	12.25 ± 0.82*
Tbet	2.30 ± 0.79	1.42 ± 0.46
Foxp3	1.74 ± 0.97	3.24 ± 0.63
IL-10	1.08 ± 0.68	2.64 ± 0.52*
TGF𝛽1	5.56 ± 0.66	6.91 ± 2.26
MCP-1	8.34 ± 3.75	3.03 ± 0.58*
IL-1𝛽	1.28 ± 0.87	0.39 ± 0.09*
TNF𝛼	1.90 ± 1.3	0.73 ± 0.18*
IL-6	2.88 ± 0.86	2.66 ± 0.61
IFN𝛾	0.63 ± 0.22	0.34 ± 0.15
IL-4	0.20 ± 0.11	0.28 ± 0.25

1Mean values ± SEM of TNBS-treated Wt and Fat-1 mice (𝑛 = 8–10/genotype). All genes were measured within the same samples and expression was normalized to ribosomal 18S (in arbitrary units). For each gene, values marked with an asterisk differ from Wt (𝑃≤0.05).