Inhibition of Age-Related Cytokines Production by ATGL: A Mechanism Linked to the Anti-Inflammatory Effect of Resveratrol

Figure 2

RSV reverts ATGL downregulation and inflammatory markers upregulation in old adipocytes. (a) RT-qPCR analysis of relative ATGL, PPARα, TNFα, and IL-6 mRNA levels in 8-day-old (8 d) and 21-day-old (21 d) adipocytes. Cells were treated with 100 μM RSV for 24 h. Values are given as means ± S.D. ^(a)𝑃<0.05 versus 8 d; ^(∗)𝑃<0.01 versus RSV untreated cells. (b) Differentiated C₂C₁₂ myotubes (4-days-old) were transfected with siRNA against ATGL [ATGL(−)] or with a scramble siRNA (Scr). Left panel: western blotting analysis of ATGL, PPAR𝛼, and TNFα in total protein extracts. Right panel: RT-qPCR analysis of relative ATGL and TNFα mRNA levels. Values are given as means ± S.D. ^(a)𝑃<0.05 versus Scr cells. (c) Left panel: western blotting analysis of ATGL and phosphoactive NFκB (pNFκB) in total protein extracts of 8-day-old adipocytes treated with 100 μM RSV for the indicated times. Right panel: the content of ATGL or pNFκB protein was quantified by densitometric analysis. Data are expressed as ATGL/β-actin or NFκB/β-actin. Values are given as means ± S.D. ^(a)𝑃<0.05 versus Ctr. GAPDH and β-actin were used as loading controls. Data are representative of at least 3 independent experiments.