Myeloid Knockout of HIF-1
α
Does Not Markedly Affect Hemorrhage/Resuscitation-Induced Inflammation and Hepatic Injury
Figure 6
Wild-type (WT) or HIF-1α knockout (KO) mice were subjected to hemorrhagic shock and resuscitation (H/R) or sham operation. Six hours after the end of resuscitation, liver tissue was harvested and western blot for the phosphorylated or nonphosphorylated p65 subunit of NF-κB and β-actin was performed (a). Lines 1, 3, and 5 were liver protein extracts from mice after sham operation, and lines 2, 4, and 6 from H/R mice (lanes 1–4: WT, lanes 5–8: KO). Representative gel from 3 experiments is shown. In (b), the ratio of phosphorylated p65 subunit of NF-κB and total protein after densitometric measurements and normalization to β-actin is represented ( versus both sham groups).
