Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors
Figure 5
Effect of infliximab on apoptotic CD16− and CD16+ TNFR1+ monocytes in diabetes mellitus type 1 patients. Peripheral blood mononuclear cells from 60 DM1 patients were incubated for 24 hours in a culture medium and then stained for visualization of the CD14, CD16, and TNF-R1 molecules. Next cellular pellet was incubated for further 15 minutes with fluorochrome-conjugated Annexin V to calculate the number of Annexin V-positive cells. The percentage of CD16− and CD16+ TNF-R1+ apoptotic cells was summarized and presented as an arithmetic mean ± S.D. Statistical significance was calculated by means of the Kruskal-Wallis Anova test.
