Research Article
P2Y12 Receptor on the Verge of a Neuroinflammatory Breakdown
Table 1
Primary antibodies employed in the study.
| | Antigen | Clone | Epitope (aa) | Target | Dilution | Source |
| | MBP | 2 | 119–131 | Mature oligodendrocytes/myelin | 1 : 100 | Chemicon | | SMI94 | SMI-94 | 70–89 | Mature oligodendrocytes/myelin | 1 : 1000 | Covance | | NG2 chondroitin sulfate proteoglycan | 132.39 | * | Oligodendrocyte precursor cells | 1 : 200 | Chemicon | CD11b
rat | OX-42 | * | Microglia/macrophages | 1 : 200 | AbD Serotec | CD11b
mouse | 5C6 | * | Microglia/macrophages | 1 : 200 | AbD Serotec | | CD68 | FA-11 | * | Macrophages/monocytes | 1 : 200 | AbD Serotec | | HLA-DP, DQ, DR (MHC II) | CR3/43 | * | Microglia/macrophages | 1 : 100 | Dako | | Integrin II/3 | (A2A9/6) | Full length | Platelets | 1 : 100 | Santa Cruz | | NeuN | A60 | * | Neurons | 1 : 200 | Millipore | | GFAP | G-A-5 | * | Astrocytes | 1 : 400 | Sigma | P2Y12 receptor
(intra1) | Polyclonal | 125–142 | P2Y12 receptor | 1 : 200 | Sigma | P2Y12 receptor
(intra2) | Polyclonal | 125–142 | P2Y12 receptor | 1 : 200–300 | Alomone | | P2Y12 receptor-ATTO-594 (intra fl) | Polyclonal | 125–142 | P2Y12 receptor | 1 : 50 | Alomone | P2Y12 receptor
mouse/rat
(c-ter) | Polyclonal | C-terminus [18] | P2Y12 receptor | 1 : 200 | Anaspec | P2Y12 receptor
human
(c-ter) | Polyclonal | 324–342 | P2Y12 receptor | 1 : 200 | Anaspec |
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CD11b: cluster of differentiation 11b; CD68: cluster of differentiation 68; fl: fluorescent at 594 nm; GFAP: glial fibrillary acidic protein; HLA: human leukocyte antigen; MBP: myelin basic protein; MHC: major histocompatibility complex; NeuN: neuronal nuclei; NG2: neural/glial antigen 2.
*Not specified in the data sheet.
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