Autophagy Upregulation and Apoptosis Downregulation in DAHP and Triptolide Treated Cerebral Ischemia

Figure 2

Apoptosis level detected by immunofluorescent labeling and TUNEL staining in each group. It can be seen from the images that both the expression of caspase-3 and TUNEL and, therefore, apoptosis, decrease in DAHP and TP. (a) The histograms show positive cells detected by TUNEL staining in each group. Values are means ± SEMs. ; ; ; n.s.: no significance. (b) The histograms showed expression of caspase-3 in each group. Values are the means ± SEMs. ; ; ; n.s.: no significance. (c) Compared with vehicle and control, the image shows more positive cells presented in MCAO, DAHP, and TP. Also MCAO owns the largest number of positive cells. (d) The images of expression levels of caspase-3 show similar change trends among each group in which both DAHP and TP decrease apoptosis. Bar = 100 μm.

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