Autophagy Upregulation and Apoptosis Downregulation in DAHP and Triptolide Treated Cerebral Ischemia

Figure 5

mTOR upregulation after MCAO was revealed under western blot and immunofluorescent staining. Western blot analysis of protein levels of mTOR and β-actin in the rat cortex derived from each group. (a) The images show that MCAO has an increase on vehicle, whereas DAHP and TP treatment groups are decreased. (b) Optical densities of respective protein bands were analyzed and normalized to the control (β-actin). Results were expressed as means ± SDs from three independent experiments. (c) From the histograms, the expression of mTOR in each group has a comparatively large fluctuation. Values are the means ± SEMs. ; ; ; n.s.: no significance. (d) The immunofluorescent staining results were consistent with those of protein level differences, mentioned above in (a). MCAO has an increasing trend. Bar = 100 μm.

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