LUBAC Formation Is Impaired in the Livers of Mice with MCD-Dependent Nonalcoholic Steatohepatitis

<div>Eight-week-old mice were fed the control or the MCD diet for 8 weeks. Their livers were removed and LUBAC formation was investigated. (a) The liver cell lysates were subjected to gel filtration. Each fraction was analyzed by western blotting with anti-HOIP, anti-HOIL-1L, or anti-SHARPIN antibody. (b) The liver cell lysates were subjected to Blue Native-PAGE without SDS, followed by western blotting with anti-HOIL-1L or anti-SHARPIN antibody. The immunoblotting data are representative of three to five independent experiments.</div>

Mediators of Inflammation

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Figure 2

Figure 2: LUBAC Formation Is Impaired in the Livers of Mice with MCD-Dependent Nonalcoholic Steatohepatitis 