Lung Epithelial TRPA1 Transduces the Extracellular ROS into Transcriptional Regulation of Lung Inflammation Induced by Cigarette Smoke: The Role of Influxed Ca²⁺

Figure 6

Cigarette smoke (CS) increases the lung expression of TRPA1 in mice. Mice were chronically exposed to air or CS for 4 weeks. (a) Representative images showing en face immunostaining with a TRPA1 antibody using whole lungs obtained from air-exposed wild-type mice and from trpa1^−/− mice. (b) Representative images showing lung section obtained from the lungs with the en face TRPA1-immunostaining of air-exposed wild-type mice and trpa1^−/− mice. (c) Representative images showing immunostaining with a TRPA1 antibody of representative lung sections obtained from air-exposed and CS-exposed wild-type mice. The specificity of the immunostaining was confirmed using an IgG-negative control. (d) Expression levels of TRPA1 in lung tissues were obtained from air-exposed and CS-exposed wild-type mice and were analyzed by Western blotting. versus the air control group in both genotypes. Data in each group are mean ± SEM from nine mice.

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