Loss of AMP-Activated Protein Kinase Induces Mitochondrial Dysfunction and Proinflammatory Response in Unstimulated Abcd1-Knockout Mice Mixed Glial Cells

Figure 3

Mitochondrial complex subunit expression and levels in wild type, Abcd1-knockout (KO), and adenosine monophosphate activated protein kinase- (AMPKα1-) deleted Abcd1-KO primary mixed glial cells. Wild type (WT) and Abcd1-KO primary mixed glial cells were cultured as described in Section 2. Abcd1-KO mixed glial cells were silenced for scrambled control (Scr) or AMPKα1 as described in Section 2. mRNA (a) and protein (b) levels of complex subunits were significantly reduced in Abcd1-KO mixed glial cells deleted for AMPKα1. (c) Densitometric ratio of mitochondrial subunit levels versus actin western blots. Results represent the mean ± SD of triplicates from two different experiments. ; ; . COM: complex, NS: nonsignificant.

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