Loss of AMP-Activated Protein Kinase Induces Mitochondrial Dysfunction and Proinflammatory Response in Unstimulated Abcd1-Knockout Mice Mixed Glial Cells

Figure 5

Adenosine monophosphate activated protein kinase- (AMPKα1-) deletion induces a spontaneous inflammatory response in Abcd1-KO mixed glial cells. Abcd1-KO mice primary mixed glial cells were plated in 6-well plates and deleted for AMPKα1 using lentiviral vector. Inducible nitric oxide synthase (iNOS) protein (a) and mRNA levels (b) were induced in Abcd1-KO mixed glial cells when deleted for AMPKα1 using lentiviral particles. Results represent the mean ± SD of triplicates from three different experiments. .

(a)

(b)