CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis

Figure 2

(a) Representative flow cytometry plots of CD4+RORγt++ and Tregs. CD4+RORγt++ were selected as the population with bright positivity for RORγt within the CD45+CD3+CD4+ cells. Tregs were selected as the CD25+FOXP3+ population within the CD45+CD3+CD4+ cells. The bar plots represent the frequency of CD4+RORγt++ and Tregs in liver, abdominal fat, and subcutaneous fat. An increase of CD4+RORγt++ cells was found in liver and abdominal fat of mice fed high fat diet (HFD), in comparison with mice fed normal diet (ND). A Treg increase was found in the subcutaneous adipose tissue. The error bars represent ±1 standard error of the mean (SEM). significant difference between mice fed HFD and ND (). (b) Fold change of leptin gene expression in liver and abdominal (AAT) and subcutaneous (SAT) adipose tissue. An increase of leptin mRNA was observed in SAT and AAT of mice fed a HFD versus mice fed a ND, with a higher increase in the SAT. Leptin gene expression was not detectable in the liver.

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