CD4+RORγt++ and Tregs in a Mouse Model of Diet-Induced Nonalcoholic Steatohepatitis
Table 2
Flow cytometry analysis.
Blood %
Liver %
Abdominal fat %
Subcutaneous fat %
(median (range))
(median (range))
(median (range))
(median (range))
ND
HFD
ND
HFD
ND
HFD
ND
HFD
CD45+
81 (42.3–78.4)
86.1 (69.2–96.3)
66.8 (54.2–78.4)
60 (48.6–71)
35.6 (26.8–73.6)
30.3 (19.8–78.6)
73.9 (32.5–95.61)
74.2 (45.8–46.62)
CD45+CD3+
14 (8.4–11.3)
9.5 (4.9–17.1)
13.6 (7.9–32.4)
18.8 (11.7–46.9)
14.8 (6–61.5)
11.7 (5.4–27.6)
33.1 (13.4–51.6)
24.7 (11.9–28.3)
CD45+CD3+CD4+
42.6 (14.6–39.7)
39.7 (30.1–52.5)
33 (27.4–35.6)
29.1 (9.2–41.4)
45.9 (40.2–56.2)
29.4 (14.7–39.8)
37.4 (28.1–41.6)
41.5 (31.1–46.6)
CD45+CD3+CD4+FOXP3+
9.6 (6.3–10.9)
10 (7.6–15.3)
5.4 (3.3–32.6)
8.5 (4.6–45)
22.8 (13.5–45.6)
16 (10.7–20.1)
8.2 (0.8–13.2)
11.7 (7.6–18.2)
Tregs (CD4+CD25+FOXP3+)
3.5 (1.7–5.5)
3 (2–4.4)
0.14 (0.06–0.6)
0.2 (0.6–3.3)
1.4 (0.6–6.7)
3 (0.6–4.3)
0.6 (0.3–1.3)
1.3 (0.8–3.5)
CD4+RORt++
1.7 (0.5–6.7)
2.2 (0.7–5)
2.4 (0.9–5.1)
9.7 (1.5–24.6)
2.3 (0.7–5.8)
8.7 (2.7–15.4)
3.6 (1–18.5)
4.5 (2–10.2)
Flow cytometric analysis of blood, liver, abdominal fat, and subcutaneous fat in mice fed high fat diet (HFD) and normal diet (ND). Cells are represented as % [(median (range)] of the gated population: CD45+CD3+ were gated on CD45+, CD45+CD3+CD4+ were gated on CD45+CD3+, CD45+CD3+CD4+FOXP3+ were gated on the CD45+CD3+CD4+, Tregs (CD45+CD3+CD4+CD25+FOXP3+) were gated on the CD45+CD3+CD4+, and CD45+CD3+CD4+RORt++ were gated on the CD45+CD3+CD4+. Statistically significant difference between mice fed HFD and ND; .
