γ-Secretase Inhibitor Alleviates Acute Airway Inflammation of Allergic Asthma in Mice by Downregulating Th17 Cell Differentiation

Figure 4

GSI administration resulted in reduced Th17 cell expansion. BALB/C mice were sensitized i.p. with OVA and challenged with OVA in the presence and absence of GSI. Splenic CD4⁺ T cells were isolated by magnetic cell sorting. Th17 cells were examined by IL-17A staining and data were analyzed by flow cytometry. Dot plots show as percent of cells positive for CD4 and IL-17A staining. Graphs representative of one of eight experiments.

(a) Sham group

(b) OVA + DMSO

(c) OVA + GSI