Ouabain Modulates Zymosan-Induced Peritonitis in Mice

Figure 8

Ouabain reduced zymosan-induced NF-κB binding activity. Electrophoretic mobility shift assay (EMSA) from peritoneal cells 4 h after injection with zymosan. Nuclear proteins (5 μg) were extracted from peritoneal cells after 4 h injection with zymosan or PBS. (a) Protein/DNA complexes positions are indicated by arrows (NF-κB) and the nonspecific band (NS) is also indicated. The respective densitometric analyses (arbitrary units (a.u.)) of the NFκB band complex presented in panel (a) are shown in graphic (b). Results were expressed as mean ± SEM and analyzed by Graphpad Prism using ANOVA with Tukey’s posttest, where all groups were compared. Results were obtained from 4 animals per group. and ^### versus PBS group and versus zymosan group.

(a)

(b)