Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells

Figure 7

DENV protease NS2B3 also induces NF-κB activation followed specifically by TNF-α expression. (a) Western blot analysis showing the expression of NS2B3 in Flag-tagged wild-type (WT) and a mutated NS2B3^S135A (S135A)-transfected RAW264.7 cells. NF-κB reporter assay (b), ELISA (c), Griess reagent (d), and western blot analysis (e) quantified the activation of NF-κB and the expression of TNF-α and iNOS/NO in transfected RAW264.7 cells. and , compared with the mock. , compared with WT NS2B3. ns: not significant. For western blot results, one set of representative data obtained from three independent experiments is shown. The relative ratio to β-actin based on densitometer quantification and analysis using ImageJ software is shown. For all experiments, the quantitative data shown represent the mean ± SD values of three independent experiments.

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