Maresin 1 Inhibits TRPV1 in Temporomandibular Joint-Related Trigeminal Nociceptive Neurons and TMJ Inflammation-Induced Synaptic Plasticity in the Trigeminal Nucleus

Figure 2

Maresin 1 potently inhibits TRPV1 but not TRPA1 current in DiI-labeled small-sized TG neurons. (a) Voltage-clamp recordings showing dose-dependent inhibition of capsaicin-induced (100 nM) TRPV1 currents by maresin 1 in DiI-labeled small-sized neurons. for each dose. (b) Dose response curve of maresin 1-induced inhibition of TRPV1 currents in DiI-labeled small-sized neurons (circle, black line) and in small-sized DRG neurons (square, gray line). Inset: IC₅₀ of TRPV1 current inhibition in TG and DRG neurons, respectively. (c) Current-clamp recording showing blockade of capsaicin-induced action potentials by maresin 1 (0.35 nM), . (d) PTX pretreatment (0.5 μg/mL, 18 h) abolishes the inhibitory effects on maresin 1 on TRPV1 currents, . (e) Maresin 1 (0.35 nM) does not inhibit AITC-induced (300 μM) TRPA1 currents ( and , -test).

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