Fas/FasL Pathway Participates in Regulation of Antiviral and Inflammatory Response during Mousepox Infection of Lungs

Figure 5

ECTV-infected macrophages and epithelial cells show different response to Fas-induced apoptosis. (a) Percentages of ECTV-infected cells in Hepa 1–6 epithelial cell cultures and bone marrow derived macrophages (BMMF) at 24 h p.i. (b) Percentage of Fas- and FasL-positive cells in epithelial Hepa 1–6 and BMMF cultures at 24 h of ECTV infection. Percentage of apoptotic (annexin V-positive) cells in epithelial Hepa 1–6 (c) and BMMF cultures (d), infected or not with ECTV and exposed or not to anti-Fas cytotoxic antibody (10 mg/mL). (e) Percentage of apoptotic cells in the cocultures of epithelial Hepa 1–6 and macrophage cells infected or not with ECTV and exposed to anti-FasL-blocking antibody (10 μg/mL). The bars represent the mean from 3 separate experiments () ± SEM. Significant differences with and .

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