RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress

Figure 2

RRM2B functions in H₂O₂-mediated DNA damage resistance in p53-deficient cells. (a) H1299 stable cells were treated with 500 μM H₂O₂ for 2 hours where applicable for immunofluorescence assay analysis with anti--H2AX antibody. (b)-(c) Two pairs of cell lines expressing control vectors, RRM2BshRNA and RRM2B expressing vector, were treated with H₂O₂ and harvested at different time points for Western blots analysis with indicated antibodies. () Image J was used to normalize -H2AX expression to GAPDH.

(a)

(b)

(c)