Helicobacter pylori Outer Membrane Vesicle Proteins Induce Human Eosinophil Degranulation via a β2 Integrin CD11/CD18- and ICAM-1-Dependent Mechanism

Figure 5

Increased CD11b expression in eosinophils stimulated with H. pylori OMVs or OMV-CM. Freshly isolated human eosinophils were stimulated with OMVs (200 μg/mL), OMV-CM (50% v/v), or control-CM (50% v/v) for 24 h. Cells were stained with mAb against CD11b (a), CD11a (b), or CD11c (c) and then analyzed using flow cytometry. Results are representative of more than five independent experiments.

(a)

(b)

(c)