Helicobacter pylori Outer Membrane Vesicle Proteins Induce Human Eosinophil Degranulation via a β2 Integrin CD11/CD18- and ICAM-1-Dependent Mechanism

Figure 7

Effects of anti-CD18 mAb on ECP released from eosinophils. (a) Eosinophils were cocultured either with unexposed control cells or with OMV-exposed primary human gastric epithelial cells (GECs) for 24 h in the presence of either anti-CD18 mAb or mouse IgG isotype control Ab. The concentration of ECP in each culture supernatant was measured by ELISA (mean ± SEM, ). (b) Human eosinophils were cocultured either with OMV-CM-exposed or control CM-exposed GECs for 24 h in the presence of either anti-CD18 mAb or mouse IgG isotype control Ab. The concentration of ECP in each culture supernatant was measured by ELISA (mean ± SEM, ). compared with unstimulated control; NS, statistically nonsignificant.

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