Anti-Inflammatory Effect of 1,3,5,7-Tetrahydroxy-8-isoprenylxanthone Isolated from Twigs of Garcinia esculenta on Stimulated Macrophage

Figure 2

Effect of TIE on NO and PGE2 production in LPS/IFNγ-stimulated RAW264.7 macrophages. (a) RAW264.7 cells were treated with TIE (3.125–25 μM) or 50 μM L-NIL for 24 h. Cell viability was measured by MTT assay. Changes in survival are represented as percentages of the control group. (b) Cells were plated at a density of 1 × 10⁵ cells/well in a 96-well plate and allowed to attach overnight. Cells were treated with the various concentrations of TIE (3.125–25 μM) or 50 μM L-NIL and incubated with LPS (100 ng/mL) and IFNγ (10 U/mL) in fresh FBS-free medium for 24 h. The nitrite production was measured by the Griess reaction. (c) Cells were treated with the indicated concentrations of TIE (12.5, 25 μM) and incubated with LPS (100 ng/mL) and IFNγ (10 U/mL) in fresh FBS-free medium for 24 h. The PGE2 concentration in cell supernatant was determined by ELISA kit. The values were presented as mean ± SEM of three independent experiments. ; versus control group; ; versus model group.

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