Anti-Inflammatory Effect of 1,3,5,7-Tetrahydroxy-8-isoprenylxanthone Isolated from Twigs of Garcinia esculenta on Stimulated Macrophage

Figure 4

Effect of TIE on proinflammatory cytokines in LPS/IFNγ-stimulated RAW264.7 cells. (a, b, c) RAW264.7 cells (1 × 10⁶ cells/dish) were treated with varying doses of TIE with IFNγ (10 U/mL) plus LPS (100 ng/mL) for 4 h. Total RNA was isolated and subjected to qRT-PCR to determine the level of IL-6 and IL-12p35/p40 mRNA. (d, e) RAW264.7 cells were treated with IFNγ (10 U/mL) plus LPS (100 ng/mL) in the presence of varying concentrations of TIE for 24 h. Total RNA was isolated and subjected to qRT-PCR to determine the level of TNF-α mRNA. Conditioned media were collected and subjected to ELISA to determine the amount of IL-6. The values (means ± SEM) were obtained from three independent experiments. , versus control group; , versus model group.

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