Decrease in Circulating Dendritic Cell Precursors in Patients with Peripheral Artery Disease

Figure 1

Gating strategy used for the identification of circulating mDCPs, pDCPs, and tDCPs by flow cytometry. (a) DCP analysis in control patients; (b) DCP analysis in patients with PAD; (c) isotype control. Region R1: white blood cells (WBCs) were separated from debris and platelets using their forward and side scatter (FSC and SSC). Region R2 was used to exclude granulocytes by SSC, B lymphocytes by CD19 staining, monocytes by CD14 staining, and dead cells by propidium iodide-staining. In regions R3 and R4, circulating mDCP and pDCP were detected according to their specific BDCA-1 and BDCA-2 staining, respectively.

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