Anti-HMGB1 Monoclonal Antibody Ameliorates Immunosuppression after Peripheral Tissue Trauma: Attenuated T-Lymphocyte Response and Increased Splenic CD11b⁺Gr-1⁺ Myeloid-Derived Suppressor Cells Require HMGB1

Figure 4

Anti-HMGB1 neutralizing mAb attenuates the expansion of CD11b⁺Gr-1⁺ MDSCs at 48 h after pseudofracture (PF). (a) Representative flow cytometric analysis and (b) graphic analysis of CD11b⁺Gr-1⁺ cell frequencies in bone marrow, blood, and spleen in control and anti-HMGB1 Ab-treated mice at 48 h after PF. versus corresponding uninjured control mouse group. Data represent means ± SEM; –8 mice per time point.

(a)

(b)