Lipid Peroxidation-Mediated Inflammation Promotes Cell Apoptosis through Activation of NF-κB Pathway in Rheumatoid Arthritis Synovial Cells

Figure 2

Activated inflammation reactions by 4-HNE treatment in MH7A synovial cells. ((a)–(c)) Real-time PCR results showing the increased mRNA levels of inflammation factors: IL1-β (a), IL-6 (b), and Tnf-α (c) in MH7A rheumatoid arthritis synovial cells after 4-HNE treatment of low concentration (5 μM) for 0~12 h. ((d)–(f)) Real-time PCR results showing the mRNA levels of inflammation factors: IL1-β (d), IL-6 (e), and Tnf-α (f) are increased (0~3 h) and then decreased (3~12 h) in MH7A rheumatoid arthritis synovial cells after 4-HNE treatment of high concentration (50 μM). Results are averages of five independent experiments. Data represent mean ± SEM. ^∗, ^∗∗, and ^∗∗∗.

(a)

(b)

(c)

(d)

(e)

(f)