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Model system | Cytokine | Ion channel | Techniques | Principle findings | Reference | EPub |
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Rabbit | TGF | ENaC | 22Na+ efflux, electrophysiology, flow cytometry, surface biotinylation, and RT-PCR | TGF decreases ENaC-mediated sodium and fluid uptake | [25] |
2014 |
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Air liquid epithelial cell culture with human CFBE41oā and transfected cells | IL6, IL8, and CXCL1/2 | CFTR, TMEM16A | Electrophysiology, immunocytochemistry, human cytokine antibody array, and cell-surface ELISA | CFTR and TMEM16A decrease secretion of IL6, IL8, and CXCL1/2 | [24] | 2012 |
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Slc26a9-deficient mice | IL13 | Slc26a9 | Electrophysiology | IL13 increases Slc26a9-mediated Clā secretion | [23] | 2012 |
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BALB/c mice | IL13 | ENaC | Electrophysiology, RT-PCR | IL13 decreases ENaC expression | [22] | 2010 |
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Human bronchial epithelial cells | IL17A | Bicarbonate transport | Electrophysiology, measurement of surface and intracellular pH | IL17A increases secretion | [21] |
2009 |
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Human nasal epithelial cells | IL13 | CFTR | Immunocytochemistry, western blot | IL13 increases CFTR expression | [20] | 2007 |
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Primary culture of rat alveolar epithelial type II cells, primary culture of human alveolar epithelial type II cells | Il1 | ENaC | 22Na+ efflux, electrophysiology, western blot, and RT-PCR | IL1 decreases ENaC expression | [18] | 2005 |
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Rat alveolar epithelial cells | TNF | ENaC | Electrophysiology, northern blot, and western blot | TNF decreases ENaC expression | [19] |
2004 |
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Primary culture of rat alveolar epithelial type II cells, primary culture of human alveolar epithelial type II cells | TGF | ENaC | 22Na+ efflux, electrophysiology, western blot, and RT-PCR | TGF decreases ENaC expression | [15] | 2003 |
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Primary culture of human bronchial epithelial cells | IL4, IL13 | CFTR, ENaC | Electrophysiology, western blot | IL4 and IL13 increase CFTR expression IL4 and IL13 decrease ENaC expression | [16] | 2002 |
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Primary culture of human bronchial epithelial cells | IFN, TNF | ENaC, CFTR | Electrophysiology, western blot, and transepithelial fluid transport | IFN decreases CFTR expression IFN decreases ENaC-mediated sodium transport TNF increases CFTR expression | [17] | 2000 |
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